Fig. 4.

Methods applied for FSHR genotyping. In the left panel, CADMA-based genotyping analysis is shown with reference peaks in the upper left corners. The three possible genotypes are characterized by distinct melting curves, which enable fast and reliable genotyping. In the mid panel, genotyping by allele specific PCR (AS-PCR) is shown along with a size marker (SM, TrackIt™ 1 KB DNA Ladder, Life technologies, Foster City, CA, USA). Each assay contain a PCR control fragment (CF; upper band) and a genotype specific fragment (GF; lower band), separated by gel-electrophoresis. The presence of a lower band indicates the genotype. In the right panel, genotyping by Sanger sequencing is shown.