Table 1.
Primer sequences
| SNP | Assay | Primer sequence (3’➙5’) | |
|---|---|---|---|
| rs6165 Thr307Ala | CADMA-based HRM assays |
Wild-type primer*: | CAGAGAGAATCTCTGAACCCTAGT |
| Mutation primer*: | CAGAGAGGGTCTCTGAGCCCTAGC | ||
| Common reverse primer: | GGCAAGAAGTTGATTATATGACTCAG | ||
| rs6166 Asn680Ser | CADMA-based HRM assays |
Wild-type primer*: | AGGGACAAGTATGTAAGTAGAACCAT |
| Mutation primer*: | AGGGACAAGTATGTGAGTGGAACCAC | ||
| Common reverse primer: | CTCTTCAGCTCCCAGAGTCACCA | ||
| rs6165 Thr307Ala | AS-PCR assays |
Wild-type primer: | GAGGATCTCTGACCCCTAGT |
| Mutation primer: | AGGATCTCTGACCCCTAGC | ||
| Control primer: | TAGCCTCAAGGGCAGGTATG | ||
| Common reverse primer: | GATGCAATGAGCAGCAGGTA | ||
| rs6166 Asn680Ser | AS-PCR assays |
Wild-type primer: | GACAAGTATGTAAGTGGAACCAT |
| Mutation primer: | GACAAGTATGTAAGTGGAACCAC | ||
| Control primer: | TTCACCCCATCAACTCCTGT | ||
| Common reverse primer: | TCCTGGCTCTGCCTCTTACA | ||
*Temperature-shifting mismatches in the primer sequences are highlighted in bold and underscored