Table 2.

Comparing results from the CADMA-based assays, AS-PCR and Sanger sequencing

	Sample no.	FSHR 307 CADMA	FSHR 680 CADMA	FSHR 307 AS-PCR	FSHR 680 AS-PCR	FSHR 307 Sequencing	FSHR 680 Sequencing
High quality DNA	1	TT	TT	TT	CT	TT	TT
	2	TT	TT	TT	TT	TT	TT
	3	TT	TT	TT	TT	TT	–
	4	TT	TT	TT	TT	–	TT
	5	TT	TT	TT	TT	TT	TT
	6	TT	TT	TT	TT	poor quality	TT
	7	CT	CT	CT	CT	CT	–
	8	CT	CT	CT	CT	CT	CT
	9	CT	CT	CT	CT	–	CT
	10	CT	CT	CT	CT	CT	CT
	11	CT	CT	CT	TT	CT	CT
	12	CT	CT	CT	CT	CT	CT
	13	CC	CC	CC	CC	CC	CC
	14	CC	CC	CC	CC	CC	CC
	15	CC	CC	CC	CC	–	CC
	16	CC	CC	CC	CC	CC	–
	17	CC	CC	CC	CC	CC	CC
	18	CC	CC	no amplification	no amplification	CC	CC
Trizol DNA	19	TT	TT	TT*	–	TT	TT
	20	TT	TT	TT*	–	poor quality	poor quality
	21	CT	CT	CT*†	–	poor quality	poor quality
	22	CT	CT	–	–	poor quality	CT
	23	CC	CC	CC*	–	CC	CC
	24	CC	CC	no amplification*	–	poor quality	poor quality

Discrepancies between assays are highlighted in red

-As sample material was limited, some of the samples could not be tested for all six genotyping assays

*The AS-PCR was performed with an additional + 6 cycles, in order to achieve results for comparison

†The AS-PCR had to be re-run in order to achieve results for comparison