FIG. 2.

H&E staining of the joint between the malleus (M) and the incus (I) (the more dorsal part) in 1-year-old (A) Coch ^+/+, D and G) Coch ^G88E/G88E, and (J) Coch ^−/− mice shows eosinophilic deposits (asterisk) in the Coch ^G88E/G88E mouse. Immunostaining with anti-cochlin antibody of (B) Coch ^+/+, E and H) Coch ^G88E/G88E, and K) Coch ^−/− (negative control) mice of sections adjacent to those with H&E staining shows cochlin staining in the incudomalleal joint of Coch ^+/+ and Coch ^G88E/G88E mice. Immunostaining for type II collagen in adjacent sections in C) Coch ^+/+, F and I) Coch ^G88E/G88E, and L) Coch ^−/− mice shows localization in the area of chondrocytes, but not in the articular joint surfaces or in eosinophilic deposits, which show cochlin staining.