FIG. 3.

H&E staining of the joint between the malleus (M) and the incus (I) (the more ventral part) of 1-year-old (A) Coch ^+/+, (D) Coch ^G88E/G88E, and (G) Coch ^−/− mice shows eosinophilic deposits (asterisk) in the Coch ^G88E/G88E mouse. Immunostaining with anti-cochlin antibody of (B) Coch ^+/+, (E) Coch ^G88E/G88E, and (H) Coch ^−/− (negative control) mice of sections adjacent to those with H&E staining shows cochlin staining in the incudomalleal joint of Coch ^+/+ and Coch ^G88E/G88E mice. Immunostaining for type II collagen in adjacent sections in (C) Coch ^+/+, (F) Coch ^G88E/G88E, and (I) Coch ^−/− mice shows localization in the area of chondrocytes, but not in the articular joint surfaces or eosinophilic deposits, which show cochlin staining.