FIG. 4.

Inhibition of microtubule dynamics or HDAC6 activity does not alter STAT3 activation in response to interleukin-6 (IL-6) treatment. (A–C) HeLa cells were treated with DMSO, paclitaxel, or nocodazole for 1 h, followed by IL-6 treatment for 30 min. (A) Phosphorylated STAT3, total STAT3, and α-tubulin were examined by immunoblotting. (B) Cells were stained with anti-STAT3 and anti-α-tubulin antibodies and DAPI. (C) The percentage of nuclear STAT3 was measured by dividing the fluorescence intensity of STAT3 in the nucleus with that in the whole cell. (D) HeLa cells treated with DMSO, tubacin, or tubastatin A for 4 h were stimulated with IL-6 for 30 min. Phosphorylated STAT3, total STAT3, acetylated tubulin, and α-tubulin were examined by immunoblotting. ns, not significant (p≥0.05).