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. Author manuscript; available in PMC: 2015 Apr 8.
Published in final edited form as: Biochemistry. 2013 Sep 23;52(40):6982–6994. doi: 10.1021/bi4005599

Figure 2. DaCld forms distinct intermediates at (A) acidic and (B) alkaline pH following rapid mixing with 3 eq PAA.

Figure 2

(A) Compound I forms at pH 6. ~15 μM DaCld (7.5 μM final) was mixed with 50 μM peracetic acid (25 μM final) in 0.2 M citrate-phosphate buffer (20 °C). \The initial spectrum is shown in black and the spectrum for the intermediate formed after ~1.5 seconds in red. Intervening spectra measured at the given times are gray. (B) Compound II or [Fe(IV)=O] + (AA▪) forms as the initial intermediate under similar conditions but at pH 8. The initial spectrum is shown in black and the spectrum for the ferryl intermediate that formed after ~300 ms in blue. Intervening spectra measured at the given times gray. Insets: visible bands (450 – 700 nm) shown on an expanded scale.