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. Author manuscript; available in PMC: 2015 Apr 8.
Published in final edited form as: Curr Protoc Cell Biol. 2012 Mar;0 15:Unit–15.17. doi: 10.1002/0471143030.cb1517s54

Table 15.17.1.

Sample Plate Setup for an Experiment with Six Time Points (0 to 60 min)

Ab dilution Eluted samplesa Lysed samplesa

1 2 3 4 5 6 7
A 1:10,000 0′ undil 5′ undil 30′ undil 0′ undil 5′ undil 30′ undil
B 1:20,000 1:2 1:2 1:2 1:2 1:2 1:2
C 1:40,000 0′ undil 5′ undil 30′ undil 0′ undil 5′ undil 30′ undil
D 1:80,000 1:2 1:2 1:2 1:2 1:2 1:2
E 1:160,000 2′ undil 15′ undil 60′ undil 2′ undil 15′ undil 60′ undil
F 1:320,000 1:2 1:2 1:2 1:2 1:2 1:2
G 1:640,000 2′ undil 15′ undil 60′ undil 2′ undil 15′ undil 60′ undil
H Blank 1:2 1:2 1:2 1:2 1:2 1:2
a

Undil, undiluted.