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. Author manuscript; available in PMC: 2015 Apr 8.

Published in final edited form as: Curr Protoc Cell Biol. 2012 Mar;0 15:Unit–15.17. doi: 10.1002/0471143030.cb1517s54

Table 15.17.1.

Sample Plate Setup for an Experiment with Six Time Points (0 to 60 min)

Ab dilution		Eluted samples^a			Lysed samples^a

	1	2	3	4	5	6	7
A	1:10,000	0′ undil	5′ undil	30′ undil	0′ undil	5′ undil	30′ undil
B	1:20,000	1:2	1:2	1:2	1:2	1:2	1:2
C	1:40,000	0′ undil	5′ undil	30′ undil	0′ undil	5′ undil	30′ undil
D	1:80,000	1:2	1:2	1:2	1:2	1:2	1:2
E	1:160,000	2′ undil	15′ undil	60′ undil	2′ undil	15′ undil	60′ undil
F	1:320,000	1:2	1:2	1:2	1:2	1:2	1:2
G	1:640,000	2′ undil	15′ undil	60′ undil	2′ undil	15′ undil	60′ undil
H	Blank	1:2	1:2	1:2	1:2	1:2	1:2

^a

Undil, undiluted.