Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2016 Apr 1.

Published in final edited form as: Arterioscler Thromb Vasc Biol. 2015 Jan 22;35(4):817–828. doi: 10.1161/ATVBAHA.114.305218

(A–B) Quantification of myocardin (A) and smooth muscle markers, smooth muscle α-actin (Acta2) and smooth muscle myosin heavy chain (Myh11; B) mRNA expression in collar-induced atherosclerosis in carotid arteries of ApoE^−/− mice (n=3). (C–D) Myocardin mRNA expression in normal human aorta and diseased carotid vessels (C; n=3) and in SMCs derived from human aorta and diseased carotid plaques (D; n=3). (E) Hematoxylin and eosin staining of aortic root sections from ApoE^−/−.Myocd^+/+ (wild type, WT) and ApoE^−/−.Myocd^+/− (Myocd^+/−) mice fed on high-fat diet for 8 weeks. Scale bar, 250 μm. (F–G) Quantification of aortic root medial (F) and plaque area (G) from WT (n=15) and Myocd^+/− (n=11) mice fed on high-fat diet. (H) Oil red O staining of aortic root sections from WT and Myocd^+/− mice fed on high-fat diet for 8 weeks. Scale bar, 250 μm. (I) Quantification of lipid rich regions by oil red O staining in the aortic root in WT (n=5) and Myocd^+/− (n=6). Data are presented as mean±s.e.m. *P<0.05 Student’s t-test. ns indicates no statistical significance.

(A–B) Quantification of myocardin (A) and smooth muscle markers, smooth muscle α-actin (Acta2) and smooth muscle myosin heavy chain (Myh11; B) mRNA expression in collar-induced atherosclerosis in carotid arteries of ApoE^−/− mice (n=3). (C–D) Myocardin mRNA expression in normal human aorta and diseased carotid vessels (C; n=3) and in SMCs derived from human aorta and diseased carotid plaques (D; n=3). (E) Hematoxylin and eosin staining of aortic root sections from ApoE^−/−.Myocd^+/+ (wild type, WT) and ApoE^−/−.Myocd^+/− (Myocd^+/−) mice fed on high-fat diet for 8 weeks. Scale bar, 250 μm. (F–G) Quantification of aortic root medial (F) and plaque area (G) from WT (n=15) and Myocd^+/− (n=11) mice fed on high-fat diet. (H) Oil red O staining of aortic root sections from WT and Myocd^+/− mice fed on high-fat diet for 8 weeks. Scale bar, 250 μm. (I) Quantification of lipid rich regions by oil red O staining in the aortic root in WT (n=5) and Myocd^+/− (n=6). Data are presented as mean±s.e.m. *P<0.05 Student’s t-test. ns indicates no statistical significance.