Phospho-site mutation S247A and inhibition of CK1δ by D4476 reduce nuclear mobility of HIF-1α in living cells.
(a) Transfected HeLa cells overexpressing the indicating HIF-1α forms tagged with GFP were processed for FRAP analysis 24 h post-transfection. Representative time-lapse images of cells are shown for each GFP-tagged protein. Circles indicate the bleached region. (b) Analysis of FRAP recoveries. Curves represent the mean corrected fluorescence intensities over time for GFP-tagged HIF-1α-ΔΝ, wt HIF-1α, HIF-1α S247A, HIF-1α S247D and wt HIF-1α in the presence of D4476, as indicated.