Fig. 5.

CK1δ inhibition increases lipid accumulation and cell proliferation under hypoxic conditions.

Treatment with D4476 (10 μΜ) of HeLa (a) or hSMB (b) cells kept under normoxia or hypoxia (1% O₂). Left: fluorescence microscope images of cells incubated under normoxia or hypoxia (1% O₂) for 24 h in the absence or presence of D4476 (10 μΜ) and stained with Nile Red to visualize lipid droplets. Right: digitized graph of cell proliferation under normoxia or hypoxia (1% O₂) in the absence or presence of D4476 (10 μΜ) after 24 h (a) or 48 h (b) treatment. Data represent the mean of three independent experiments performed in triplicate and expressed, as percent of the initial number of cells at time zero ± SEM.