Table 1.
Measured and kinetic parameter estimates of FRAP experiments.
| easyFRAP |
Model-based analysis |
|||||
|---|---|---|---|---|---|---|
| GFP-HIF-1α construct | N | t1/2 (s) | Mobile fraction | Diffusion coefficient (μm2/s) | Bound fraction | Residence time (s) |
| ΔN | 18 | 0.15 ± 0.07 | 0.95 ± 0.04 | 47.1 ± 5.1 | 0.01 ± 0.01 | – |
| wt | 14 | 0.29 ± 0.10 | 0.87 ± 0.11 | 21.6 ± 16 | 0.09 ± 0.04 | 19.2 ± 6.3 |
| S247D | 9 | 0.35 ± 0.19 | 0.93 ± 0.03 | 12.5 ± 6.9 | 0.12 ± 0.05 | 15.4 ± 5.4 |
| S247A | 13 | 0.74 ± 0.68 | 0.82 ± 0.16 | 13.6 ± 11.8 | 0.18 ± 0.13 | 20.1 ± 6.6 |
| wt + D4476 | 15 | 1.87 ± 2.04 | 0.70 ± 0.21 | 10.8 ± 11.6 | 0.28 ± 0.14 | 20.8 ± 6.6 |
EasyFRAP: values of half-maximal recovery time (t1/2) and mobile fraction for different GFP-HIF-1α constructs, processed as described in Materials and methods.
Model-based analysis: kinetic parameter estimates of FRAP recovery curves for different GFP-HIF-1α constructs. Numerical values correspond to the mean ± standard deviation of different recovery curves corresponding to different cells (N represents the number of cells analyzed in each condition).