FIG. 2.

oxLDL induced the expression of Sestrin-2 in RAW264.7 cells. (A, B) RAW264.7 cells were stimulated with oxLDL at various concentrations for 24 h. (C, D) RAW264.7 cells were stimulated with oxLDL at indicated doses (50 μg/mL) for varying periods of time. Protein levels of Sestrin-2 were determined by western blot analysis. mRNA levels of Sestrin-2 were determined by real-time polymerase chain reaction (RT-PCR). Data are presented as the mean±SEM of at least three independent experiments. *p<0.05 versus nontreated control.