Table 4. Comprehensive immunophenotype comparison of RPCI-WM1, MWCL-1 and BCWM.1.
| RPCI-WM1 | MWCL-1 | BCWM.1 | |||||
|---|---|---|---|---|---|---|---|
| WM associated antigens | Fluorochrome | % Gated | MESF | % Gated | MESF | % Gated | MESF |
| CD10 | PE | 8.8 | 1661.6 | 2.5 | 1057.6 | 4.5 | 1216.1 |
| CD11c | APC | 3.5 | 318.3 | 2.1 | 231.0 | 6.8 | 285.6 |
| CD19 | APC | 9.0 | 394.5 | 97.8 | 30981.5 | 98.0 | 16617.6 |
| CD20 | APC | 1.1 | 0.0 | 99.8 | 92499.2 | 98.9 | 66979.7 |
| CD22 | PECY5 | 20.0 | 958.8 | 63.7 | 2819.3 | 97.3 | 15665.7 |
| CD23 | APC | 8.9 | 372.8 | 99.4 | 110591.5 | 94.8 | 38999.8 |
| CD25 | APC | 40.3 | 849.9 | 22.1 | 546.6 | 20.3 | 394.5 |
| CD27 | PE | 4.3 | 1374.9 | 8.5 | 1343.1 | 7.3 | 1661.5 |
| CD28 | APC | 99.6 | 21596.2 | 16.0 | 427.1 | 12.0 | 329.2 |
| CD38 | PECY5 | 99.5 | 413090.1 | 44.4 | 2107.9 | 91.5 | 9367.8 |
| CD40 | PE | 10.9 | 1661.6 | 79.8 | 5994.1 | 82.8 | 8448.2 |
| CD52 | FITC | 4.8 | 2183.6 | 65.5 | 7335.7 | 20.9 | 3158.5 |
| CD56 | FITC | 2.1 | 1814.0 | 2.2 | 1537.6 | 2.2 | 1353.8 |
| CD138 | PE | 87.7 | 14582.8 | 98.3 | 70921.5 | 34.9 | 3719.5 |
| CD45RO | PE | 89.4 | 7268.5 | 4.5 | 1311.3 | 12.5 | 1470.3 |
| CD79b | PE | 1.6 | 1311.4 | 24.6 | 2109.1 | 2.0 | 491.1 |
| FMC7 | FITC | 1.1 | 1583.7 | 63.6 | 8659.9 | 45.4 | 5827.4 |
| KAPPA | APC | 99.1 | 10789.6 | 99.5 | 33782.1 | 26.7 | 633.3 |
| All other antigens | Fluorochrome | % Gated | MESF | % Gated | MESF | % Gated | MESF |
| CD5 | PECY5 | 3.9 | 689.1 | 30.9 | 1317.3 | 4.0 | 288.5 |
| CD13 | PE | 4.1 | 1534.1 | 6.1 | 1438.5 | 17.2 | 1629.7 |
| CD14 | APC | 27.6 | 600.9 | 17.3 | 448.8 | 18.1 | 405.3 |
| CD16 | FITC | 2.8 | 1767.9 | 2.0 | 1537.6 | 2.7 | 1583.6 |
| CD24 | PE | 15.8 | 2109.2 | 15.2 | 1949.1 | 7.3 | 1343.1 |
| CD30 | PE | 1.8 | 1279.6 | 50.5 | 4984.5 | 95.3 | 52990.2 |
| CD32 | PE | 0.9 | 710.6 | 0.9 | 553.6 | 3.2 | 553.6 |
| CD34 | PE | 4.8 | 1406.7 | 4.7 | 1216.1 | 5.4 | 994.3 |
| CD35 | PE | 1.4 | 1216.1 | 2.2 | 1025.9 | 8.5 | 1184.3 |
| CD39 | PE | 98.6 | 30127.2 | 99.3 | 98989.4 | 99.2 | 61333.3 |
| CD43 | FITC | 97.9 | 27244.7 | 98.7 | 40373.8 | 94.4 | 31498.3 |
| CD45 | FITC | 98.1 | 24447.2 | 84.8 | 12746.5 | 91.4 | 15611.4 |
| CD45RA | APC | 48.7 | 1087.7 | 50.6 | 1184.8 | 89.7 | 5577.0 |
| CD54 | FITC | 98.3 | 22809.7 | 92.9 | 21078.1 | 97.2 | 43972.5 |
| CD62L | FITC | 2.5 | 1814.0 | 3.6 | 1675.7 | 1.5 | 1216.2 |
| CD66b | PCPCY5.5 | 97.9 | 3592.2 | 93.8 | 3440.3 | 77.7 | 2273.3 |
| CD69 | PE | 3.1 | 1311.4 | 46.0 | 3396.2 | 50.3 | 3525.5 |
| CD70 | PE | 99.2 | 49943.4 | 99.8 | 82511.4 | 97.2 | 64254.8 |
| CD73 | FITC | 2.7 | 1860.2 | 7.5 | 2044.9 | 0.2 | 6062.7 |
| CD80 | PE | 2.4 | 1279.6 | 99.7 | 39474.6 | 3.8 | 804.9 |
| CD86 | APC | 99.6 | 10598.5 | 87.9 | 4830.0 | 98.3 | 12327.9 |
| CD90 | FITC | 2.6 | 1767.9 | 17.9 | 2739.9 | 10.1 | 1998.6 |
| CD101 | APC | 41.7 | 795.8 | 80.9 | 3632.8 | 35.3 | 535.7 |
| CD105 | PE | 6.4 | 1565.9 | 79.0 | 5114.6 | 12.5 | 0 |
| CD110 | PE | 96.7 | 6712.5 | 97.9 | 9597.9 | 64.6 | 3687.1 |
| CD111 | PE | 5.2 | 1470.4 | 66.0 | 4335.0 | 63.7 | 4594.6 |
| CD117 | APC | 4.2 | 329.2 | 2.5 | 274.7 | 5.2 | 274.7 |
| CD123 | PE | 85.9 | 7301.2 | 89.8 | 12928.3 | 79.0 | 9597.9 |
| CD127 | PE | 2.3 | 1247.8 | 4.0 | 1152.6 | 7.2 | 1279.5 |
| CD133 | PE | 4.8 | 1629.7 | 3.5 | 1247.8 | 7.4 | 1502.2 |
| CD134 | PE | 80.7 | 4237.7 | 67.3 | 4562.1 | 92.3 | 9828.1 |
| CD135 | PE | 6.5 | 1597.8 | 5.0 | 1343.1 | 8.2 | 1406.7 |
| CD137 | PE | 4.7 | 1502.2 | 6.8 | 1406.7 | 52.4 | 3428.5 |
| CD154 | PE | 1.3 | 1152.7 | 2.4 | 1025.9 | 4.2 | 1120.9 |
| CD184 | PECY5 | 98.3 | 19115.2 | 8.6 | 740.5 | 88.6 | 9618.4 |
| CD197 | PE | 1.7 | 1216.1 | 41.5 | 3234.8 | 36.6 | 2719.2 |
| CD202b | PE | 12.1 | 1821.2 | 14.4 | 1885.1 | 9.0 | 1279.5 |
| CD243 | PE | 16.3 | 2173.3 | 14.4 | 1949.1 | 26.3 | 1725.4 |
| CD252 | PE | 16.6 | 1885.2 | 45.7 | 4952.0 | 38.4 | 5863.7 |
| CD268 | PE | 33.5 | 2526.3 | 94.9 | 32241.5 | 97.4 | 24368.1 |
| CD272 | PE | 24.1 | 1981.1 | 96.1 | 31805.0 | 88.8 | 27111.1 |
| CD278 | FITC | 4.4 | 2137.4 | 5.9 | 2183.6 | 6.6 | 2461.5 |
| CD279 | APC | 39.0 | 774.2 | 23.5 | 274.7 | 24.6 | 579.1 |
| CD309 | PE | 11.8 | 1853.2 | 9.3 | 1629.7 | 8.7 | 1438.5 |
| CD338 | APC | 12.1 | 470.6 | 5.4 | 329.2 | 6.8 | 285.6 |
| HLADR | FITC | 1.6 | 1721.8 | 99.6 | 149806.8 | 99.6 | 105318.4 |
| LAMBDA | PE | 4.1 | 1534.1 | 4.4 | 1311.3 | 98.1 | 20863.7 |