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. 2015 Apr 8;10(4):e0122038. doi: 10.1371/journal.pone.0122038

Fig 3. Species identification of Leishmania from sand flies collected from trails in the Xakriabá Indigenous Reserve, Brazil, during the study period.

Fig 3

Sand flies were grouped in pools of up to ten specimens of the same species, locality, and date, and ITS1 PCR-RFLP was performed with total DNA extracted from these pools. The figure represents an ethidium bromide-stained 4% agarose gel in which the amplicons were submitted to electrophoresis. Lanes: MW, molecular weight marker—100 bp; lanes 1–4, positive controls of Le. amazonensis (IFLA/BR/67/PH8), Le. braziliensis (MHOM/BR/75/M2903), Le. infantum (MHOM/BR/74/PP75), Le. guyanensis (MHOM/BR/75/M4147) respectively; 5–14, phlebotomine positive pools.