Gene expression analysis with and without partial thyroidectomy. (A) Venn diagram of up- and down-regulated genes in SP versus non-SP cells. Int: intact thyroid, PTx: partial thyroidectomized thyroid. Stc1, Tm4sf1, and Vwf are among genes commonly up-regulated in both intact and partial thyroidectomized thyroids. (B) Quantitative RT-PCR (qRT-PCR) analysis of Stc1, Tm4sf1, and Vwf genes in SP and non-SP cells without (Int Thy) and with (PTx Thy) partial thyroidectomy, using two rounds amplified RNAs. The relative expression levels of SP cells were separately expressed using those of corresponding non-SP cells as 1. Therefore the levels of Int Thy and PTx Thy cannot be directly compared. *p<0.05, **p<0.01.