FIG. 4.

Analysis of STC1 expression in thyroid cancers. (A) qRT-PCR analysis of STC1 expression in human thyroid carcinoma derived FTC133, FTC236, FTC238, RO82-W-1, and 8305C cells (left). The relative expression was expressed using that of FTC133 as 1. qRT-PCR analysis of STC1 expression in the RO82-W-1 thyroid carcinoma cell line and in normal human thyroid tissue (right). The relative expression was expressed using that of RO82-W-1 as 1. The results are shown as the mean±SD from quadruple analysis. The same experiments were repeated at least two more times, and similar results were obtained. *p<0.05, ****p<0.001 based on the value of FTC133. NS, not significant by One-way ANOVA. (B) A representative Western blotting of STC1 protein expression using a whole cell lysate of the aforementioned cells. The Western blot was done four times. Cell lysates expressing human STC1 by transfection of Stc1 expression plasmid (2 and 4 μg) was used as a positive control, while those carrying a vector only were used as a negative control (lysates purchased from Novus). The multiple bands detected were largely accounted for by glycosylation of STC1 (80). GAPDH was used as a loading control of cell lysate.