FIG. 3.

SOAE inhibits VCAM1 and MCP-1 expression in HUVECs. HUVECs were pretreated with SOAE followed by addition of 10 ng/mL TNF-α. Cells were incubated for 24 h at the end of which total cellular RNA was extracted and reverse transcribed. Real-time PCR analysis was performed to analyze mRNA levels of VCAM1 (A) and MCP-1 (B). PCR products are represented in panel (C). Western blot analysis to determine VCAM1 protein expression was carried out (D). Similar experiments were carried out with HUVECs treated with mm-LDL and SOAE. Gene expression of VCAM1 (E) and MCP-1 (F) were analyzed. Results are representative of three experiments. Error bars represent SEM. *P<.05; **P<.01. HUVECs, human umbilical vein endothelial cells; LDL, low-density lipoprotein; MCP-1, monocyte chemoattractant protein-1; mm-LDL, minimally modified LDL.