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. 2015 Apr 1;18(4):393–402. doi: 10.1089/jmf.2014.0139

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Copyright 2015, Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition

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FIG. 6. — Differential regulation of expression of scavenger receptors and reverse cholesterol transport genes by SOAE. RAW cells were treated with increasing concentration of SOAE following which expression of SR-A1 and CD36 was analyzed. While SR-A1 mRNA expression was found to decrease with increasing concentration of SOAE (A), CD36 mRNA expression markedly increased (B). The protein expression of SRA1 and CD36 (C) corroborated with the real-time PCR results. The effect of SOAE on reverse cholesterol transport genes was analyzed by real-time PCR (D). Using HepG2-LXR reporter system the effect of SOAE on GFP expression was assayed; the leftmost panel are the bright field images; the middle panel represent fluorescence images; and rightmost panel are the merged images (E). Luciferase activity (F) was also assayed in these cells. Results are average of three independent repeats. *P<.05; **P<.01. LXR, liver X receptor; SR-A1, scavenger receptor A1.