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TNF-α and IL-6 ELISA for mice serum samples. TNF-α and IL-6 levels were measured by ELISA from serum of mice as described in methods. As shown in (A), pretreatment of mice with 50 μg and 100 μg SOAE (**P<.003) showed a significant reduction in LPS-induced TNFα levels compared with LPS-treatment alone. A similar result was observed with IL-6 at 10 μg SOAE (**P<.004). However, a dose-dependent increase in IL-6 (B) levels was observed at concentrations higher than 10 μg SOAE.
