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Dot-ELISA assay of monoclonal antibodies (MAbs). PEDV-containing cell culture fluid and cell cultures were spotted onto nitrocellulose membranes. The supernatants from hybridoma 3H4 (A), 5D7 (B), or SP2/0 (C) myeloma cells were used as primary antibodies. Distinct spots with (A) and (B) indicated a positive result, while fuzzy spots with (C) and the lack of a spot with Vero as the antigen indicated a negative result.
