Fig 4. Localization of truncated RPS17 proteins containing amino acid mutations within putative nuclear localization signals.

(A) Truncated RPS17 fragments that bestowed nuclear import to triple eYFP were mutated to determine the critical residues responsible for nuclear import. This schematic depicts the amino acid sequences of 1–13, 43–61, and 31–50 (capable of nuclear import) along with basic amino acids mutated to alanine or threonine. (B) Confocal microscopy images of RPS17 truncations with and without basic amino acids mutated to alanine or threonine. Lysines, 10/11, lysine and arginine 48/49, and lysines 44/45 were important for nuclear import in the context of amino acids 1–13, and 43–61 respectively, however, 44/45 was dispensable in the context of amino acids 32–50. Scale bars on merged images represent 10 μm.