Table 1. Oligonucleotide primer sequences used for PCR amplification and mutation of RPS17 and triple eYFP (restriction sites are underlined).

Primer ID	Primer Sequence (5’→3’)	Tm (°C)	Function
Spk383	CATCATCTCGAGATGGGCCGCGTTCG	64.9	RT-PCR RPS17
Spk384	CATCATCCGCGGCCAAACAGGTCCCCGAGG	70.1	RT-PCR RPS17
Spk407	CATCATAAGCTTTGCTATCTTGTTGCGGAGC	61.2	RPS17 39–51
Spk419	GACGCAAATGGGCGGTAG	56.7	eYFP OE PCR
Spk420	CCTCGCCGGACACGC	60.1	eYFP OE PCR
Spk421	CCACACGAACACACGCGTG	59.6	RPS17 KR32/33AA
Spk422	CACGCGTGTGTTCGTGTGG	59.6	RPS17 KR32/33AA
Spk425	CATCATCTCGAGATGAACGACTTCCACACGAACAAG	63.4	RPS17 39–51
Spk426	CATCATAAGCTTGTCGTTGCCCAGGCGC	65.2	RPS17 1–38
Spk427	CATCATCTCGAGATGCAGGTTACGTCACGCATCTG	64.9	RPS17 51–135
Spk434	CATCATTGTACACCATGGTGAGCAAGGGCGAG	65.0	eYFP (x2)
Spk436	CATCATGCGGCCGCCTCGTCCATGCCGAGAGT	71.6	eYFP (x2)
Spk467	GTATGGCTGATTATGATCTAGAGTCG	54.5	eYFP (x3)
Spk484	CATCATGCGGCCGCCGGAATGGTGAGCAAGG	70.3	eYFP (x3)
Spk505	CATCATAAGCTTCCGGGCCGCCTTCTTCAC	65.5	RPS17 1–13
Spk506	CATCATCTCGAGATGAGCAAAAAGCTCCGCAAC	63.5	RPS17 44–61
Spk507	CATCATAAGCTTAATTCGCTTCATCAGATGCG	59.4	RPS17 44–61
Spk512	CAAAACCGTGGCGGCGGCGGCC	70.9	RPS17 KK10/11AA
Spk513	GGCCGCCGCCGCCACGGTTTT	70.9	RPS17 KK10/11AA
Spk514	CCAGCGCAGCGCTCCGCAACAAG	67.7	RPS17 KK44/45AA
Spk515	CTTGTTGCGGAGCGCTGCGCTGG	67.7	RPS17 KK44/45AA
Spk516	CATCATCTCGAGATGGCGGCCCGGGTCATC	68.0	RPS 11–38
Spk517	CATCATTTCGAAGCTGGGGATAATGGCGATCTC	63.1	RPS 11–38