Fig. 1.

Anti-lysine succinylation immunoblots showing that different carbon sources altered succinylation profiles of Mtb H37Rv. Coomassie blue staining of protein lysates from A 30 days and B 60 days of cultures. Coomassie blue staining was used for the loading control. Western blotting analysis of lysine succinylation level in protein lysates from C 30 days and D 60 days of cultures with a pan anti-succinyllysine antibody. Specificity of succinylation signals was validated by succinylated or non succinylated BSA assay.