Fig. 1.

Proteome analysis of mouse skeletal muscle. A, Protein lysates (triplicate) from mouse triceps muscle and C2C12 were digested on FASP filter and peptides were separated on an OFFGEL fractionator. Each fraction was analyzed by LCMS on a Q Exactive mass spectrometer. Representative spectrum from skeletal muscle, B, and C2C12 myotubes, C, at MS and MSMS levels. D, Number of protein identification without match between runs (black) and with match between runs option (gray). E, Total number of proteins identified in skeletal muscle and C2C12 cells.