Table 7. Intra-Operator Variability (Duplicates) for the Trizol LS RNA Extraction and Preamplicification Protocol.
| Colorectal Advanced Adenoma + Comparison (n = 32) | ||||
|---|---|---|---|---|
| microRNAs | Mean CT Value Duplicate A (±SD) | Mean CT Value Duplicate B (±SD) | |ΔCT| † | p-value |
| miR-374 | 25.98 (±1.80) | 26.00 (±1.93) | 0.02 | 0.9518 |
| miR-142-3p | 28.48 (±2.77) | 28.74 (±3.05) | 0.27 | 0.6177 |
| miR-523 | 20.48 (±2.08) | 20.21 (±1.71) | 0.27 | 0.4240 |
| miR-374-5p | 29.47 (±3.53) | 29.81 (±3.61) | 0.34 | 0.6186 |
| miR-376c | 32.27 (±2.63) | 31.68 (±2.33) | 0.58 | 0.3265 |
| miR-27a | 28.62 (±2.25) | 29.24 (±2.99) | 0.62 | 0.2216 |
| miR-520d-5p | 35.61 (±1.90) | 35.24 (±1.77) | 0.36 | 0.4806 |
| miR-122 | 28.88 (±2.40) | 29.11 (±2.82) | 0.23 | 0.6814 |
| miR-485-3p | 29.32 (±3.06) | 29.44 (±3.32) | 0.12 | 0.8320 |
| miR-21 | 26.97 (±2.58) | 26.96 (±2.81) | 0.01 | 0.9833 |
| miR-218 | 35.90 (±2.38) | 35.83 (±2.08) | 0.07 | 0.9206 |
† |ΔCT| = absolute value (mean cycle threshold value for duplicate 1 for the miRNA of interest—mean cycle threshold value for duplicate 2 for the miRNA of interest.