Table 8. Differences in ΔCT between operators with Trizol LS RNA extraction and pre-amplification (inter-operator variability).
| Colorectal Advanced Adenoma (n = 16) + Comparison (n = 16) | ||||||
|---|---|---|---|---|---|---|
| Unpaired t-test—Cycle Threshold = 0.03 | ||||||
| Operator 1 | Operator 2 | p-value ¥ | Mean |ΔΔCT|*(±SD) | |||
| microRNAs | Mean ΔCT † (±SD) | # miRNA Not Expressed | Mean ΔCT † (±SD) | # miRNA Not Expressed | ||
| miR-374 | -1.95 (±2.53) | 0 | -1.53 (±2.39) | 0 | 0.4974 | 1.69 (±1.32) |
| miR-142-3p | 0.84 (±1.99) | 0 | 1.23 (±3.06) | 0 | 0.5478 | 1.69 (±1.83) |
| miR-523 | -7.01 (±3.11) | 0 | -7.78 (±2.17) | 0 | 0.2551 | 1.45 (±1.45) |
| miR-374-5p | 2.02 (±2.59) | 2 | 2.83 (±2.85) | 3 | 0.2577 | 1.69 (±1.73) |
| miR-376c | 5.83 (±2.21) | 11 | 5.48 (±2.71) | 11 | 0.6490 | 2.42 (±1.42) |
| miR-27a | 1.25 (±1.46) | 1 | 1.50 (±1.77) | 5 | 0.5580 | 1.29 (±1.23) |
| miR-520d-5p | 8.57 (±3.39) | 14 | 7.00 (±2.73) | 17 | 0.1587 | 2.05 (±2.03) |
| miR-122 | 2.41 (±4.23) | 5 | 2.21 (±3.98) | 8 | 0.8632 | 1.82 (±1.86) |
| miR-485-3p | 1.48 (±2.35) | 0 | 1.34 (±2.50) | 0 | 0.8182 | 1.45 (±1.45) |
| miR-21 | -1.83 (±2.60) | 0 | 0.05 (±2.01) | 0 | 0.0019 | 2.40 (±1.82) |
| miR-218 | 8.01 (±2.88) | 13 | 8.93 (±2.62) | 19 | 0.3650 | 1.77 (±1.79) |
| Total Average | 1.79 (±1.63) | |||||
† ΔCT = miRNA of interest cycle threshold value—U6 cycle threshold value.
* |ΔΔCT| = Absolute Value [Operator 1 ΔCT—Operator 2 ΔCT].
¥ = p value of mean ΔCT operator 1 vs operator 2.
Bold = statistically significant.