Table 10. Differences in ΔCT between operators with Qiagen miRNeasy with RNA Carrier and No Pre-amplification (Inter-Operator Variability).
| Colorectal Advanced Adenoma (n = 16) + Comparison (n = 16) | ||||||
|---|---|---|---|---|---|---|
| Unpaired t-test—Cycle Threshold = 0.03 | ||||||
| Operator 1 | Operator 2 | p-value ¥ | Mean |ΔΔCT|*(±SD) | |||
| microRNAs | Mean ΔCT † (±SD) | # miRNA Not Expressed | Mean ΔCT † (±SD) | # miRNA Not Expressed | ||
| miR-374 | -1.80 (±1.75) | 0 | -1.69 (±1.99) | 0 | 0.8151 | 0.70 (±0.78) |
| miR-142-3p | -7.16 (±1.57) | 0 | -6.60 (±1.81) | 0 | 0.1910 | 0.91 (±0.89) |
| miR-523 | -3.21 (±2.82) | 0 | -2.12 (±2.92) | 0 | 0.1339 | 1.38 (±1.02) |
| miR-374-5p | -3.27 (±1.94) | 0 | -2.33 (±1.87) | 0 | 0.0529 | 1.27 (±1.06) |
| miR-376c | -0.04 (±1.74) | 0 | 0.05 (±1.98) | 0 | 0.8475 | 0.76 (±0.91) |
| miR-27a | -6.12 (±1.56) | 0 | -5.52 (±1.79) | 0 | 0.1579 | 0.98 (±1.03) |
| miR-520d-5p | 5.54 (±2.54) | 0 | 6.27 (±2.91) | 1 | 0.2925 | 1.20 (±1.01) |
| miR-122 | -2.69 (±2.96) | 0 | -2.05 (±3.58) | 0 | 0.4387 | 1.11 (±1.10) |
| miR-485-3p | -2.60 (±2.02) | 0 | -1.31 (±2.26) | 0 | 0.0191 | 1.50 (±1.01) |
| miR-21 | -7.59 (±1.75) | 0 | -6.69 (±1.85) | 0 | 0.0500 | 1.36 (±1.00) |
| miR-218 | -3.73 (±2.28) | 0 | -2.90 (±2.23) | 0 | 0.1460 | 1.09 (±1.06) |
| Total Average | 1.12 (±0.99) | |||||
† ΔCT = miRNA of interest cycle threshold value—U6 cycle threshold value.
* |ΔΔCT| = Absolute Value [Operator 1 ΔCT—Operator 2 ΔCT].
¥ = p value of mean ΔCT operator 1 vs operator 2.
Bold = statistically significant.