Table 2. Cytotoxicity of different cell lines with bacterial culture supernatant of netF-positive (JFP726, JFP728) and cpb-positive (NCTC3110) C. perfringens strains.
| Cytotoxicity titer 2 | ||||
|---|---|---|---|---|
| Cell lines 1 | Origin of cell lines | NCTC3110 | JFP728 (Equine) | JFP726 (Canine) |
| EO | Equine | 16 | 128 | 128 |
| MDCK | Canine | N 3 | 4 | 4 |
| A72 | Canine | 4 | 8 | 8 |
| MDBK | Bovine | N | 2 | 2 |
| PK15 | Pig | N | 4 | 4 |
| 208F | Rat | 2 | 2 | 2 |
| NIH 3T3 | Mouse | N | N | N |
| CaCo2 | Human | N | 2 | 2 |
| LMH | Chicken | 2 | N | N |
| Vero | Monkey | N | N | N |
1EO: Equine ovarian cell line, MDCK: Madin Darby canine kidney cell line, A72: Canine fibroblasts cell line, MDBK: Madin Darby bovine kidney cell line, PK15: Porcine kidney cell line, 208F: Rat Fischer fibroblast cell line, N1H 3T3: Mouse embryo fibroblast cell line, CaCo2: Human colon epithelial cell line, LMH: Primary chicken hepatocellular carcinoma epithelial cell line, Vero: African green monkey kidney cell line.
2 Cytotoxicity was evident after 8 h of exposure of 2-fold dilution series of the filter-sterilized culture supernatants up to 1:1024 to different cells. The titer is the final dilution showing > 2+ cell toxicity.
3 No cytotoxicity.