Table 6. ELISA cross-reactivity and cytotoxin neutralizing titers to recombinant toxins rNetE, rNetF and rNetG in horses immunized with different rNet-NusA proteins.
| ELISA | ||||||
|---|---|---|---|---|---|---|
| Antigens | Horses 1 | Neutralizing anti-serum titers 2 | rNetE | rNetF | rNetG | rNetB |
| rNetE | 1 | 643 | 102400 | 6400 | 12800 | 51200 |
| 2 | 128 | 25600 | 1600 | 1600 | 12800 | |
| rNetF | 3 | 25600 | 12800 | 204800 | 12800 | 6400 |
| 4 | 6400 | 6400 | 102400 | 12800 | 12800 | |
| 5 | 3200 | 12800 | 204800 | 12800 | 6400 | |
| rNetG | 6 | 64 | 6400 | 12800 | 51200 | 12800 |
| 7 | 128 | 51200 | 51200 | 102400 | 51200 | |
1Immunization: Horses 1, 2, rNetE-NusA; horses 3–5, rNetF-NusA; horses 6, 7, rNetG-NusA
2The neutralization of cytotoxicity by polyclonal antibody was performed with the EO cell line
3The neutralizing antibody titer was that showing an inhibition of 2+ or greater