| pJIR750 |
E. coli—C. perfringens shuttle vector, CmR
|
J.I.Rood, Monash University |
| pNetF07 |
pJIR750CmR containing netF gene and 250 bp of its upstream region |
This study |
| pMTL007 |
Inducible clostridial expression vector for expression of ClosTron, containing Erm RAM, ColE1, pCB102, CmR
|
[62] |
| pMTLnetE |
pMTL007 containing intron retargeted to C. perfringens netE (sense insertion at 883–884 bp) |
This study |
| pMTLnetF |
pMTL007 containing intron retargeted to C. perfringens netF (antisense insertion at 152–153 bp) |
This study |
| pMTLnetG |
pMTL007 containing intron retargeted to C. perfringens netG (antisense insertion at 583–584 bp) |
This study |
| pET-28a |
E. coli expression vector. PT7, KanR, ori pBR322, ori f1, lacI, T7, Tag N-terminal 6xHis, C-terminal 6xHis |
(Novagen, Gibbstown, NJ) |
| pET28::netE
|
pET28a containing the DNA fragment amplified by PCR using primers netE-F-EcoRI and netE-R- HindIII |
This study |
| pET28::netF
|
pET28a containing the DNA fragment amplified by PCR using primers netF-F- EcoRI and netF-R-XhoI |
This study |
| pET28::netG
|
pET28a containing the DNA fragment amplified by PCR using primers netG-F-BamHI and netG-R- XhoI |
This study |
| pET43.1a |
E. coli expression vector. PT7, AmpR, ori pBR322, ori f1, lacI, T7, Tag N-terminal Nus, C-terminal 6xHis |
(Novagen, Gibbstown, NJ) |
| pET43::netE
|
pET43.1a containing the DNA fragment amplified by PCR using primers netE-F- EcoRI and netE-R- HindIII |
This study |
| pET43::netF
|
pET43.1a containing the DNA fragment amplified by PCR using primers netF-F- EcoRI and netF-R-XhoI |
This study |
| pET43::netG
|
pET43.1a containing the DNA fragment amplified by PCR using primers netG-F-BamHI and netG-R- XhoI |
This study |
