Figure 3. CARs with a constitutive growth phenotype display a unique gene signature.

Cytokines, perforin and granzyme expression. Microarray analysis comparing cytokine expression of c-Met IgG4 (green), CD19 CD8-α (red), CART19 (blue) CARs and untransduced (orange) T cells at baseline and on days 6, 22 and 24 of culture; only the c-Met IgG4 culture was analyzed on day 24 because the other cultures were terminated due to cell death. No exogenous cytokines were added to the culture media. Normalized absolute log₂ gene expression intensities are plotted for IFNγ, TNFα, IL17A, IL2, IL3, IL4, GM-CSF, IL10, IL13, Granzyme B and Perforin, The design of the CAR constructs is shown in Supplemental Figure S1.