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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Cancer Immunol Res. 2015 Jan 19;3(4):356–367. doi: 10.1158/2326-6066.CIR-14-0186

Figure 6. A,B and C. Transgene expression levels are sufficient to convey the constitutive CAR growth phenotype.

Figure 6

In vitro proliferation of human CD4+ T cells following 5 days of anti-CD3 plus anti-CD28 stimulation and lentiviral transduction with c-MET-expressing CARs under the control of the indicated promoter. CMV(1) and CMV (2) represent replications of lentiviral vector production in the same human donor. A) Population doublings were determined for both CMV and EF-1α driven c-MET CAR cells. After ∼12 days in culture, CMV-c-MET CAR cells were unable to sustain proliferation and died, while EF-1α c-MET CAR T cells continue to proliferate. B) Mean cell volume (MCV) was also determined. The CMV-c-MET CAR T cells decreased in cell size after 10 days, indicative of the cells resting down. C) Comparison of the level of expression between CARs expressed with the CMV and EF-1α promoters is shown at day 6 post-transduction. The mean fluorescence intensity is indicated. The design of the CAR constructs is shown in Supplemental Figure S1.