Table 1.
| Antibody (Dilution) | Immunogen | Catalog#/Manufacturer/ Lot# | Specificity Controls | |
|---|---|---|---|---|
| Validation | Staining Pattern | |||
| Calretinin (1:1000) | Rat calretinin | Millipore AB1550 lot LV148604 Polyclonal (goat), RRID: AB_90764 | Immunoblot: Manufacturer, Hackney et al., 2003; | Type II hair cells and afferents as reported (Desai et al., 2005; Pujol et al. 2014) |
| Calretinin (1:1000) | Rat calretinin | Chemicon AB5054 lot LV1552190 Polyclonal (rabbit), RRID: AB_2068506 | Immunoblot: Manufacturer, Hackney et al., 2003; Liu and Davis, 2014. | Type II hair cells and afferents as reported (Desai et al., 2005; Pujol et. al 2014) |
| CGRP (1:500) | amidated carboxyl 7aa of rat αCGRP | Mu33; a kind gift from Ian Dickerson Polyclonal (rabbit) | Immunprecipitation: Rosenblatt and Dickerson, 1997 | Efferent fibers and varicosities as reported (Demêmes et al., 2001; Luebke et al., 2014) |
| CGRP (1:500) | Mature rat full length αCGRP | JH84; A kind gift from Ian Dickerson Polyclonal (rabbit) | Immunprecipitation: Rosenblatt and Dickerson, 1997 | Efferent fibers and varicosities as reported (Demêmes et al., 2001; Luebke et al., 2014) |
| Choline Acetyltransferase (1:100) | Human placental enzyme | Chemicon AB144P lot JC1618187 Polyclonal (goat) RRID:AB_2079751 |
Immunoblot: Lee et al., 2007); Wibowo et al. 2009. | Efferent fibers and varicosities as reported (Ohno et al., 1993; Kong et al., 1994) |
| Choline Acetyltransferase (1:500) | Chick brain | A kind gift from Dr. Epstein Polyclonal (rabbit) RRID: AB_2314177 |
Immunoblot: Johnson and Epstein, 1986 | Efferent fibers and varicosities as reported (Ohno et al., 1993; Kong et al. 1994; Zidanic 2002) |
| Myosin VIIA 10 μg/ml | aa880-1077 from the tail region of human Myo7A | Proteus #25-6790, Polyclonal (rabbit) RRID:AB_10015251 |
Immunoblot: Hasson et al., 1995 | Type I and II hair cells as reported (Hasson et al. 1997; Duncan et al., 2006) |
| Myosin VIIA* (1:100) | aa927-1203 from human Myo7A | DSHB #138-1, Monoclonal (mouse) RRID:AB_2282417 |
Immunoblot: Soni et al., 2005 | Type I and II hair cells as reported (Hasson et al. 1997; Duncan et al., 2006) |
| Synapsin I (1:500–1000) | Bovine Synapsin I (mixture of Ia and Ib) | Chemicon AB1543 lot LV1463637 Polyclonal (rabbit), RRID:AB_2200400 | Immunoblot: Kopeikina et al., 2013. | Punctated labeling of efferent varicosities (Favre et al., 1986; Holstein et al., 2005) |
| SV2** 1:200–10,000 | Synaptic vesicle (Ommata electric organ) | DSHB SV2 conc. Ascites, Monoclonal (mouse), RRID:AB_2315408 | Immunoblot: Buckley and Kelly 1995; Wibowo et al. 2009 | Punctated labeling of efferent varicosities (Layton et al., 2005; Wibowo et al., 2009). |
| Syntaxin (1:1000) | Synaptosomal plasma membrane fraction from adult rat hippocampus | Sigma #S0664 Clone HPC-1 lot 019K4834, Monoclonal (mouse) RRID:AB_477483 |
Immunoblot: Barnstable et al. 1985; Inoue et al., 1992; see also manufacturer data sheet | Punctated labeling of efferent varicosities (Hennig and Cotanche, 1998) |
*
Generated by Dana Jo Orten
**
Developed by K. Buckley. Both were obtained from the Developmental Studies Hybridoma Bank developed under the auspices