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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Mol Microbiol. 2015 Feb 4;96(2):249–262. doi: 10.1111/mmi.12935

Figure 3. The FasX sRNA negatively regulates the abundance of pili on the surface of serotype M2 GAS.

Figure 3

Western blot analysis showing FasX inhibition of pilus expression in M2 GAS. Cell wall proteins from exponential phase cultures were isolated from the indicated strains, separated by SDS-PAGE, and used in Western blot analysis with sera that reacts against the major M2 pilus protein. The membrane was stained for use as a loading control. Densitometry analysis of the Western data was performed and is presented as percent pilus expression relative to the parental strain containing empty vector.