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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Mol Microbiol. 2015 Feb 4;96(2):249–262. doi: 10.1111/mmi.12935

Figure 5. FasX base-pairs to the 5’ regions of M2.113 mRNA in serotype M2 GAS M6.tee6 mRNA in M6 GAS, and M28.fctA mRNA in M28 GAS.

Figure 5

RNA:RNA electrophoretic mobility shift assays confirming base-pairing between FasX and the 5′ end of (A) M2.113 mRNA in M2 GAS, (B) M6.tee6 mRNA in M6 GAS, and (C) M28.fctA mRNA in M28 GAS. A biotin-labeled RNA probe was incubated with wild-type FasX (FasX RNA; lanes 2–4 and 6–9), a FasX mutant in which six of the complementary nucleotides had been deleted (FasXΔ72–77 RNA; lane 5), unlabeled RNA probe (lanes 6–8), or unlabeled yeast tRNA (lane 9).