. Author manuscript; available in PMC: 2016 Apr 15.

Published in final edited form as: J Immunol. 2015 Mar 9;194(8):3594–3600. doi: 10.4049/jimmunol.1403234

Table IV.

Relative expression levels of HLA class-I loci measured by mass spectrometry.

Sample type	Amount detected (fmol)				Level relative to HLA-C
Sample type	HLA-A	HLA-B	HLA-C	HLA-E	HLA-A	HLA-B	HLA-E
B cell line	2,888	2,759	521	32	6	5	0.06
Normal PBL (donor 1)	2,905	2,503	582	23	5	4	0.04
Normal PBL (donor 2)	3,424	3,137	726	31	5	4	0.04

Amounts detected are medians from the multiple peptides used to quantitate each HLA locus. For each donor and the cell line, expression levels shown are from a single representative experimental replicate. Ranges of expression relative to HLA-C in these replicates were: B cell line, n=8, HLA-A (5.5-6.2), HLA-B (4.8-5.6), HLA-E (0.06-0.07); PBL donor 1, n=4, HLA-A (4.3-5.0), HLA-B (3.7-4.3), HLA-E (0.03-0.06); PBL donor 2, n=4, HLA-A (4.3-5.0), HLA-B (3.6-4.3), HLA-E (0.03-0.05).