Figure 3. Cpn-positive macrophages and neutrophils were reduced in Wsh compared to WT.

WT and Wsh mice were infected with 2×10⁶ IFU Cpn intratracheally. Mice were sacrificed either 3 or 5 days post Cpn infection. BAL cells and lung cells were collected at day 3 (A, B) or day 5 (B, D) and stained with anti-Cpn, anti-CD11c, anti CD11b, anti-F4/80, and anti-Ly6G for flow cytometric analysis as before. A) Representative histograms for Cpn staining in gated populations (see Supplementary Figure 1 for gating strategy). B) The percent of Cpn+ cells in the BALF and Lung at day 3. C) Representative histograms for Cpn staining in gated populations on day 5. D) The percent of Cpn+ cells in the BALF and Lung at day 5. For A and B, the experiment was performed twice n=5 (WT) n=6 (wsh). For C and D, the experiment was performed twice n=4 (WT) n=4 (wsh). Data shown is a representative experiment. Data are mean ±SD. Significance of differences was determined by Mann-Whitney. *P<0.05.