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. Author manuscript; available in PMC: 2016 Apr 15.
Published in final edited form as: J Immunol. 2015 Mar 9;194(8):3840–3851. doi: 10.4049/jimmunol.1402685

Figure 4. Cromolyn reduces inflammatory cell infiltration into the airspace and bacterial number in the lung.

Figure 4

WT mice were treated with cromolyn (3 mg/kg body weight) prior and post Cpn infection (2×106 IFU) and sacrificed 5 days post infection. (A) Total and differential cell counts in BALF. Cell types in BALF were determined by modified Wright-Giemza staining. (B) Single cells from lung were collected through enzymatic procedure and analyzed by flow cytometry for differential cell counts. (C) Chemokine concentrations in BALF and the lung homogenates were determined by ELISA. (D) Cpn IFU in the lungs were quantified as described in Materials and Methods. For A–D, this experiment was performed twice n=5 (Cpn) n=6 (Cpn + Cro). Data shown is a representative experiment. Data are mean ±SD. Significance of differences was determined by Mann-Whitney. *P<0.05, ***P<0.001.