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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Feb 14;1853(6):1370–1394. doi: 10.1016/j.bbamcr.2015.01.025

Figure 16.

Figure 16

X-band HYSCORE spectra of biotin synthase paramagnetic intermediate grown in E. coli with natural abundance arginine (top) and (guanidino-15N2)-l-arginine (bottom). The 15N coupling observed in the bottom HYSCORE spectrum corresponds to 15N labeling of the Arg260 residue. Reprinted with permission from Fugate, et al. [153] Copyright 2012 American Chemical Society.