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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Mech Dev. 2015 Feb 23;136:40–52. doi: 10.1016/j.mod.2015.02.003

Fig. 4.

Fig. 4

A poly(A) tail enhances β-complex assembly on the β-globin 3′UTR. (A) Assembly of the β-complex is enhanced on a polyadenylated β-globin 3′UTR. R-EMSA analyses were conducted on β-globin 3′UTR RNAs that either did (+) or did not (−) contain an 18-nt poly(A) tail, using cytoplasmic extract prepared from K562 cells. Sense (S) and antisense (AS) DNA competitors verify the identity of the β-complex (arrow); 20% of each undigested probe was loaded as a control for transcript intensity and integrity. (B) Polyadenylation enhances β-complex assembly on β-globin mRNA 3′UTRs in primary human erythroid cells. As in panel A, using cytoplasmic extract purified from d10 EPO-induced CD34+ erythroid progenitor cells.