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. Author manuscript; available in PMC: 2016 Apr 27.
Published in final edited form as: Vaccine. 2015 Mar 1;33(18):2159–2166. doi: 10.1016/j.vaccine.2015.02.055

Fig. 3.

Fig. 3

(A). T cell antigenicity of C. trachomatis (Ct) membrane protein antigens (left panel) and other identified T cell antigens (right panel) in immune C57BL/6 mice identified by IFN-γ ELISPOT assay. Mice were transcervically infected with 2 × 107 IFU live C. trachomatis serovar D. Fourteen or 21 days after infection, the splenocytes were harvested and stimulated in vitro for 20 h with 1 μg/ml individual Chlamydia proteins or 5 × 105 IFU/ml heat killed-EB as a positive control. The results represent the average of duplicate wells and are expressed as the means ± SEM of Chlamydia Ag-induced IFN-γ -secreting cells per 106 splenocytes for groups of five mice. (B). T cell antigenicity of C. muridarum (Cm) Pmps and MOMP in immune C57BL/6 (left panel) and C3H (right panel) mice identified by IFN-γ ELISPOT assay. Mice were intravaginally infected with 1500 IFU of C. muridarum. Three weeks later, splenocytes were harvested and stimulated in vitro for 20 h with 1 μg/ml individual Chlamydia proteins or heat killed-EB as a positive control.