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. Author manuscript; available in PMC: 2015 Apr 9.
Published in final edited form as: Integr Biol (Camb). 2015 Apr 7;7(4):435–446. doi: 10.1039/c4ib00281d

Fig. 6. SC-35 domain descriptors can identify alkaline phosphatase expression on micropillars within 3 days.

Fig. 6

hMSCs were cultured on a subset of micropillars (termed TopoA - TopoD) derived from a much larger library of topographies. (A) Cells show no substantial qualitative differences in SC-35 organization after 3 days in culture, although at 14 days (B) hMSCs cultured in the same conditions displayed significant differences in percentage of alkaline phosphatase positive cells. 3 day SC-35 domain descriptors are capable of identifying differences between the topography culture conditions, as seen by (C) PCA plots of demarcating distinct groupings between the conditions with largest difference in ALP positive cells (TopoA versus TopoD), as well as intermediate ALP positive conditions (TopoB versus TopoC), and (D) decision tree classification analysis of PCA-derived principal components. For analyzing SC-35 organization, between 41 and 56 cells were analysed for each condition. Scale bar = 10 μm. * indicates statistical significance (p < 0.01) with respect to TopoA (most osteogenic) condition.