Skip to main content
. Author manuscript; available in PMC: 2016 May 15.
Published in final edited form as: Mol Cell Endocrinol. 2015 Mar 9;407:52–56. doi: 10.1016/j.mce.2015.03.002

Figure 1. SMRT Deficiency Increases GR-Mediated Transactivation.

Figure 1

GRE-Luciferase and pSG5-GR plasmids were transfected into wild-type (black bars) or SMRT −/− (gray bars) mouse embryonic fibroblasts, along with a CMV-b-galactosidase plasmid to assess transfection efficiency. 5 hrs after transfection, the indicated amount of dexamethasone was added (0 nM – 100 nM). 24 hrs after transfection, luciferase activity was assessed. Transfection efficiency was controlled for by measuring beta-galactosidase activity. Dexamethasone statistically increased luciferase expression in WT MEFs. Dexamethasone had a significantly larger effect in SMRT −/− MEFs. The statistical evaluation noted in the figure compares luciferase expression in WT versus that in SMRT −/− cells for each dose of dexamethasone. * p<0.05 (compared to WT cells), using 2 tailed 2 sample equal variance student t test. Three experiments were performed in triplicate.