Total RNA from epididymal fat pads was extracted and cDNA synthesis was performed. Experiments were performed as described in the manual for SybrGreen (Quanta) with minor modifications. Each cDNA sample in triplicate was subjected to three individual PCR analyses using primers specific to Gilz, Lipin, or 18s RNA. Gene expression corrected using 18s data, and 1.0 was defined as the relative gene expression in WT adipose tissue. Data is expressed as fold change from WT. * p<0.05, using 2 tailed 2 sample equal variance student t test of relative gene expression when compared with WT. Data was collected from at least three experiments, and each reaction was performed in triplicate.