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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Clin Ther. 2015 Feb 27;37(3):498–504. doi: 10.1016/j.clinthera.2015.02.004

Figure 2. Workflow for the protein sandwich immunoassay.

Figure 2

Microspheres are coupled with capture antibodies, incubated with a saliva sample and then biotinylated detection antibodies, and finally a streptavidin-conjugated fluorescent probe is added to the complex for a quantified readout.