Figure 1.
Acute and chronic exposure of Candida albicans to lactic, acetic, propionic, and butyric acid: (A) Overall scheme of the transcriptional profiling experiment. C. albicans overnight cultures were diluted in fresh media every 24 hr and samples for RNA extraction were collected 4 hr after each dilution. (B) OD600nm of the culture was measured at 4 hr and 24 hr after C. albicans inoculation in media supplemented with the indicated acids (all adjusted to pH 5.5 with the addition of NaOH) and compared with untreated control cultures (De Man, Rogosa, and Sharpe medium [MRS] at pH 6.1). n = 5; *P < 0.05 **P < 0.01. OD, optical density.