Figure 4.

Weak organic acids reduce intracellular iron concentrations in Candida albicans: (A−B) C. albicans cells were grown under the indicated conditions, and the relative concentration of iron was determined in the culture supernatant (A) or in the cell pellet (B) using a colorimetric assay. MRS, De Man, Rogosa, and Sharpe medium; NAT, nourseothricin. (C) C. albicans sfu1Δ mutant and isogenic control (wild type; WT) cells (Noble et al. 2010) were grown under the indicated conditions and the relative concentration of iron was determined in the cell pellet using the same colorimetric assay. n = 3; *P < 0.05; **P < 0.01.