Figure 2.

Regulation of immunity and stress response–related genes by H1. (A) Multiple immune response and stress response–related genes are upregulated in H1-depleted larval salivary glands. Real-time RT-PCR assays of transcripts were performed in H1-depleted and control salivary glands. Fold changes are calculated as a ratio of signals for H1-depleted samples to those for control samples. Values are normalized to the expression of rp49, are representative of three independent experiments, and are presented in a logarithmic scale. SDs are shown as error bars. Transcripts for Act5C, Gapdh1, and βTub60D are shown as controls. (B) Antimicrobial peptide (AMP) genes are not substantially affected by H1 knockdown in salivary glands. Real-time RT-PCR assays of transcripts were performed in H1-depleted and control salivary glands, analyzed, and presented as in (A). Transcript for βTub60D is shown as a control. (C) NFκB-family transcription factors Dorsal (dl), Dorsal-related immunity factor (Dif), and Relish (Rel) are not coordinately regulated on H1 depletion in salivary glands. Real-time RT-PCR assays of transcripts were performed in H1-depleted and control salivary glands, analyzed, and presented as in (A). Black, white, and gray bars indicate transcripts that are significantly upregulated, downregulated, or not affected, respectively, in the H1 knockdown.